fluorescein test

An immunofluorescence assay(IFA) mediated by monoclonal antibody 1D12 was developed to detect antigen of Porcine reproductive and respiratory syndrome virus in fr

应用淋巴细胞杂交瘤技术,最后一次免疫后第三天取其脾细胞与SP2/0细胞在聚乙二醇作用下融合,通过酶联免疫吸附试验(ELISA)和间接免疫荧光试验(IFA)筛选,得到2株稳定分泌抗PRRSV NVDC-JXA1株特异性抗体的杂交瘤细胞,命名为1D12、5H5。